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Protein G Bestaresin 4 HF
Protein G Bestaresin 4 HF media can be used to separate antibodies or antibody FC fragments of different species from cell culture or cell extract.
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Product | Code no. | Pack size |
Protein G Bestaresin 4HF | AA201401 | 25ml |
AA201402 | 100ml | |
AA201403 | 500ml | |
AA201404 | 1L | |
AA201405 | 5L | |
AA201406 | 10L |
Specifications
Matrix
Highly crosslinked 4% agarose
Average Particle Size
90μm
Ligand
E.coli rProtein G
Ligand Density
2mgrProteinG/ml media
Crosslink Method
CNBr activation
Binding Capacity
>20mg human IgG/ml media
pHStability
3-9(Working) 2-10(CIP,Short Period)
Pressure/Flow Rate
Matrix150-250cm/h,1bar(0.1MPa);
Height 25cm,Diameter 5cm;
Column:BXK 16/20 height10cm
Sample:Mice IgG ascites sample
Flow Rate:2ml/min
Binding Buffer:PBS pH7.4
Elution Buffer:100mM Gly,pH2.7
Protein G 4 HF purification of mice IgG
Reduce SDS-PAGE electrophoresis
1.LMW marker;2. Mice ascites; 3-7. FT;8. Elution(10X dilution);9. Elution