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021-2023 0623
AT Protein A Diamond Plus
Self-developed completely new anti-alkali Protein A, good tolerance for 0.5M NaOH.
Completely avoid the use of expensive and corrosive cleaning agents such as guanidine hydrochloride. Directly clean using 0.1-0.5M NaOH , greatly reducing medium usage cost.
Human IgG 10% dynamic binding capacity increase to 50mg / ml, greatly reducing the cost of the medium
High flow rate, large pore diameter, new Diamond matrix, greatly improving production efficiency.
If you want to know the price and details of products, please contact us.
Buy Hotline: 021-58953301 / 021-2023 0623
| Product | Code no. | Pack size |
| AT Protein A Diamond Plus | AA203601 | 25ml |
| AA203602 | 100ml | |
| AA203603 | 500ml | |
| AA203604 | 1L | |
| AA203605 | 5L | |
| AA203606 | 10L |
Matrix
Highly crosslinked agarose
Average Particle Size
75μm
Ligand
Basic rProtein A (E.coli)
Crosslink Method
Epoxy Chemistry
Dynamic Binding Capacity
＞55mg Human IgG /ml media
Chemical Stability
Protein A is stable in most commonly used buffer
pH Stability
3-12(Working)，0.1-0.5M NaOH(CIP，Short Method)
Recommend Flow Rate
500cm/h
Storage Condition
PBS，20％Ethanol，4-8℃
Column：
Alkali Resistant Protein A Diamond，EzScreen
Sample：human IgG cell culture
Binding Buffer：
PBS(20mM PB， 0.15M NaCl，pH7.4)
Elution Buffer：
100mM Gly, pH2.7
Notice: Eluted buffer need to be neutralizated with 1M Tris (pH9.0)
Alkli Resistant Protein A purification: IgG SDS-PAGE（12%）SDS-PAGE
1：LMW Marker； 2-4：FT； 5：Wash； 6：Elution