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Mixmode cation exchanger
Bestaresin Diamond MMC
MMC Bestaresin 6HF
MMC is complex cationic ligand. The medium with the ligand can capture and purify protein under high salt concentration without conductivity dilution.
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| Product | Code no. | Pack size |
| Diamond MMC | AI200901 | 25ml |
| AI200902 | 100ml | |
| AI200903 | 500ml | |
| AI200904 | 1L | |
| AI200905 | 5L | |
| AI200906 | 10L | |
| AI200907 | 20L |
Ion exchange type
Bestaresin Diamond MMC
MMC Bestaresin 6HF
Dynamic capacity
Bestaresin Diamond MMC electrical conductance 30mS/cm, 28mg BSA/ml media
MMC Bestaresin 6HF electrical conductance 30mS/cm, 28mg BSA/ml media
Recommended operational flow velocity
Bestaresin Diamond MMC <1000cm/h
MMC Bestaresin 6HF 50-400cm/h
Matrix
Bestaresin Diamond MMC Highly Cross-linked agarose
MMC Bestaresin 6HF Highly Cross-linked agarose 6%
Average particle size
Bestaresin Diamond MMC 75μm
MMC Bestaresin 6HF 90μm
pH stability
2-12(Working)，2-14(CIP，Short-term)
Chemical stability
In all commonly used stable aqueous buffer：8M carbamide、6Mguanidine hydrochloride、70% alcohol、1M NaOH、1M acetic acid
Storage Conditions
20% Ethanol
Storage temperature
4-30℃
Media：Bestaresin Diamond MMC
Column：BXK 50/30
Sample：300ml某重组蛋白毕赤酵母发酵液
Equilibration buffer：0.4M NaCl，20mM PB，pH6.5
Elution buffer：1.5M NaCl，20mM PB，pH6.5
Bestaresin Diamond MMC纯化某重组蛋白
Bestaresin Diamond MMC Purification of a recombinant proteinSDS-PAGE
1：低分子量蛋白Marker； 2：原样；3-6：流穿；7-8：清洗；9：洗脱